Relationship between sterol synthesis and DNA synthesis in phytohemagglutinin-stimulated mouse lymphocytes.
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چکیده
منابع مشابه
Relationship between sterol synthesis and DNA synthesis in phytohemagglutinin-stimulated mouse lymphocytes.
Incubation of peripheral blood or isolated lymphocytes of C57L/J mice with phytohemagglutinin stimulated the incorporation of thymidine into DNA of lymphocytes as they transformed into large lymphoblasts. DNA synthesis began after about 24 hr of incubation and reached a peak at 48 hours. The de-novo synthesis of sterols from acetate was stimulated much earlier, at 4 hr of incubation, and the ra...
متن کاملCharacterization of DNA excreted from phytohemagglutinin-stimulated lymphocytes
The DNA released into the culture medium after phytohemagglutinin (PHA) stimulation of human peripheral blood lymphocytes has been purified and characterized. It is double stranded, sediments at 7-8S in alkaline sucrose, and has a Tm determined optically and by thermal elution from hydroxyapatite that is substantially lower than that found for lymphocyte cell DNA. Media DNA contains a major com...
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The association of a human genetic deficiency of adenosine deaminase activity with combined immunodeficiency prompted a study of the effects of adenosine and of inhibition of adenosine deaminase activity on human lymphocyte transformation and a detailed study of adenosine metabolism throughout phytohemagglutinin-induced blastogenesis. The adenosine deaminase inhibitor, coformycin, at a concentr...
متن کاملA method for counting phytohemagglutinin-stimulated lymphocytes.
I T HAS BEEN SHOWN that peripheral i)lood lynlphocvtes undergo trainsformation and mitosis in vitro when cultured in the presence of plwtohemagglutinin ( PHA).’4 Little information has been reported on the numl)er of lymphocytes which survive and transform during the culturing interval. It has been very difficult to enumerate lymphocytes in cultures containing PFIA I)ecause the agglutinated ery...
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By JEROME I. BRODY AND HENRY D. SOLTYS T HE PUBPOSE of this study was to delineate the durability and persistence of immunologic recall in the normal peripheral blood lymphocyte using methods which might reflect the importance of the manner by which imniunologic information is acquired. This was done in the present investigation with the technic of bacterial adherence,”2 in which circumferentia...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1975
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.72.5.1950